We narrowed to 23,247 results for: ica

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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S269D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-OPHN1

Plasmid

#221310

Purpose

Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

OPHN1 (pLxIS) (Ophn1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-FES

Plasmid

#221307

Purpose

Expression of FES (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FES (pLxIS) (Fes Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-TBK1

Plasmid

#221331

Purpose

TBK1 protein overexpression in Sf9 insect cells

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TBK1 (Tbk1 Mouse)

Tags

6xHis-TEV-2xGST-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGM79

Plasmid

#220171

Purpose

CRISRPi guide RNA against MARCHF5

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

MARCHF5 (sgRNA) (MARCHF5 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

Plasmid

#220078

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Expression

Mammalian

Mutation

deltaTMD

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-1-SAC1deltaTMD-Fis1tail

Plasmid

#220077

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Expression

Mammalian

Mutation

deltaTMD

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-STReTCh (Force Inert)

Plasmid

#216710

Purpose

Expresses HaloTag-RGD-STReTCh Force Inert Control in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-RGD-STReTCh

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(8G)-mCherry

Plasmid

#206950

Purpose

Mammalian expression of heterobifunctional SasG linkers (8G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (8G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mt SSB

Plasmid

#205057

Purpose

SSB1 protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

SSB1 (SSB Human)

Expression

Bacterial

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-Golgi-bapaUGAc

Plasmid

#201675

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

Golgi-bapaUGAc

Tags

Golgi leading sequence

Expression

Mammalian

Promoter

CMV SP6

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mNeon

Plasmid

#186598

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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H1.224.M.luc

Plasmid

#193705

Purpose

Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated PolIII H1 promoter (H1.224) with the TATA box mutated

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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