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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Tcf/Lef:H2B-GFP

Plasmid

#32610

Depositor

Anna-Katerina Hadjantonakis

Article

Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

Insert

Tcf/Lef:H2B-GFP

Promoter

hsp68

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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