164,829 results

1. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-cdh1

   Plasmid

   #11596

   Depositor

   Marc Kirschner

   Article

   Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

   Insert

   cdh1 (FZR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJR103

   Plasmid

   #187242

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCherryGFP-CoV2-WT

   Plasmid

   #177618

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-16

   Plasmid

   #172746

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. L0-I35 p35S-XVE-pEstradiol GG0-GG1

   Plasmid

   #195904

   Purpose

   Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   p35S-XVE-LexA-p35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 ZoAl N-1F

   Plasmid

   #203763

   Purpose

   For expression of N-flag tagged ZoAl R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3505

   Plasmid

   #144981

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF12 (KLF12 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPT107

   Plasmid

   #85525

   Purpose

   Repressilator with triple reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   mKate2
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4b Q61H

    Plasmid

    #83140

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61H

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. EKAREN5-gl

    Plasmid

    #225958

    Purpose

    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

    Depositor

    Toru Hiratsuka

    Article

    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

    Insert

    EKAREN5-gl
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

    Plasmid

    #204808

    Purpose

    Bacterial co-expression of Dengue2 NS2B and NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue2 NS2B (POLY Dengue2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pENTR221-mCherry

    Plasmid

    #79509

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    mCherry RFP
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-NLGN3 WT

    Plasmid

    #59318

    Purpose

    Expression plasmid of HA-tagged wildtype human Neuroligin 3

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid

    #176278

    Purpose

    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0058

    Plasmid

    #143007

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MAF (MAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

    Plasmid

    #192184

    Purpose

    Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

    Plasmid

    #192183

    Purpose

    Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge