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Addgene

We narrowed to 13,226 results for: GAN

Showing: 1741 - 1770 of 13226 results
  1. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pCK354

    Plasmid
    #129698
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799
    Depositor
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pEA02

    Plasmid
    #172193
    Purpose
    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Insert
    attL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pEA03

    Plasmid
    #172194
    Purpose
    Suicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Insert
    attR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-sCAG-GluClβ.CreON

    Plasmid
    #196996
    Purpose
    Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.
    Depositor
    Insert
    GluClβ -YFP
    Use
    AAV and Cre/Lox
    Tags
    YFP
    Expression
    Mutation
    Promoter
    short CAG
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pJN601

    Plasmid
    #59790
    Purpose
    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker
    Depositor
    Inserts
    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pClneoFH-let60 G12V

    Plasmid
    #66860
    Purpose
    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells
    Depositor
    Insert
    Let-60 (let-60 Nematode)
    Use
    Tags
    FLAG-His6
    Expression
    Mammalian
    Mutation
    Glycine 12 is mutated to valine
    Promoter
    CMV
    Available since
    Aug. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pHW1387

    Plasmid
    #229880
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pHW1385

    Plasmid
    #229879
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Depositor
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Use
    Tags
    mScarlet-I-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pHW1392

    Plasmid
    #229878
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Insert
    Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pHW1391

    Plasmid
    #229877
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Psnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Depositor
    Insert
    Psnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Use
    Tags
    mScarlet-I-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pHW1223

    Plasmid
    #229875
    Purpose
    gene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Insert
    LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pHW1134

    Plasmid
    #229870
    Purpose
    gene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Insert
    cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pAAV-sCAG-GluClα.CreON

    Plasmid
    #196995
    Purpose
    Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.
    Depositor
    Insert
    GluClα -Cerulean
    Use
    AAV and Cre/Lox
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    short CAG
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1741 - 1770 of 13226 results