We narrowed to 4,128 results for: pCMV mammalian

1. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

   Plasmid

   #207344

   Purpose

   Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Δ37, Δ115-133, Δ204-217

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p169 pCMV/betaAc-EGFP/beta-gal

   Plasmid

   #8388

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP beta-gal
   

   Use

   Cre/Lox; Beta-gal

   Expression

   Mammalian

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Promoter

   pCMV and T7

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Flag-YAP-S163/164A

   Plasmid

   #33089

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S163A, S164A

   Promoter

   CMV

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCMV superYFP-AURKA K162M-mTurq2

   Plasmid

   #157773

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV sfGFP-CHMP2B-201-213

   Plasmid

   #232016

   Purpose

   Expression of the CHMP2B VPS4-binding region attached to sfGFP.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV CHMP2B-L4D F5D-3XHA

   Plasmid

   #232003

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

   Plasmid

   #201466

   Purpose

   Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 111-136

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

    Plasmid

    #201467

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 137-160

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

    Plasmid

    #201468

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 202-230

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

    Plasmid

    #201469

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 287-316

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197027

    Purpose

    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enRhCas12f1
    

    Expression

    Mammalian

    Mutation

    L270R

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197024

    Purpose

    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized OsCas12f1
    

    Expression

    Mammalian

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197025

    Purpose

    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized RhCas12f1
    

    Expression

    Mammalian

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV5 MYC-Smurf2 (FF29/30A) C716A

    Plasmid

    #24605

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, C716A

    Available Since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

    Plasmid

    #24606

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, Y459A

    Available Since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

23. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid

    #221448

    Purpose

    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3

    Depositor

    Omar Akbari

    Inserts

    wtCas9-NG
    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-HA_N-human Prkd2 without kinase domain

    Plasmid

    #138411

    Purpose

    Express Prkd2 without kinase domain in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    Prkd2 without kinase domain (PRKD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    kinase domain deleted from human Prkd2

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP

    Plasmid

    #241135

    Purpose

    Expresses Anti-HA frankenbody-mScarlet3 and HaloTag-tdMCP to track mature and nascent HA-tagged proteins and MS2-tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP
    

    Tags

    HaloTag and mScarlet3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-Flag YAP127/128/131/381A

    Plasmid

    #27378

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    S127/128/131/381A

    Available Since

    March 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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36. FLAG-ADA add back mutant pCMV5

    Plasmid

    #17554

    Depositor

    Domenico Accili

    Article

    Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

    Insert

    FLAG-FoxO1-ADA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA-resistant Foxo1 by replacing 3 residues (un…

    Available Since

    Oct. 17, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pCMV6-MPP8-delta2-441-10xHis

    Plasmid

    #194181

    Purpose

    Expresses MPP8-delta2-441_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta2-441

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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38. pCMV6-MPP8-delta442-860-10xHis

    Plasmid

    #194182

    Purpose

    Expresses MPP8-delta442-860_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta442-860

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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39. pCMV6-MPP8-delta600-728-10xHis

    Plasmid

    #194183

    Purpose

    Expresses MPP8-delta600-728_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta600-728 (4xANK deletion)

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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40. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid

    #197500

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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