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Plasmid#63754Purpose3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.DepositorInsertehxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1783-end
Plasmid#46071DepositorInsertMap7-1783-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-856-1351
Plasmid#46069DepositorInsertMap7-856-1351 (Map7 Mouse)
UseUnspecifiedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-2A-ZsGreen
Plasmid#231985PurposeEmpty backbone for expression of GoI with Bi-cistronic expression ZsGreenDepositorInsert2A-ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Adeno EA
Plasmid#64071PurposeAdenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4DepositorInsertU6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
UseAdenoviralTagsFLAG-Cas9ExpressionMutationPromoterU6 and CBhAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags3XFLAG-Cas9ExpressionMammalianMutationPromoterCBh; U6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1
Plasmid#194278PurposeExpresses gRNA and dSaCas9-KRAB from lentiviral vectorDepositorInsertshumanized dSaCas9 KRAB T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-GFP-WPRE-pA
Plasmid#192063PurposeExpression of BFP, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKWT
Plasmid#216540PurposeThis retroviral plasmid expresses the human wild type PTK2DepositorInsertPTK2 (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationwild type sequencePromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-sesRNA-GFP-WPRE-pA
Plasmid#192065PurposeExpression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for Cheta
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-tTA2-WPRE-pA
Plasmid#192070PurposeExpression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-ADAR2-sesRNA-GFP-W3SL
Plasmid#192069PurposeExpression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.DepositorInsertsesRNA for mouse Ctip2
UseAAVTagsExpressionMammalianMutationPromoterCbhAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLM-CMV-R-Cre
Plasmid#27546PurposeLentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.DepositorInsertmCherry_P2A_Cre recombinase
UseCre/Lox and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176628PurposeTF A self-activation cassette in MultiFate-2.3DepositorInsert14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only