We narrowed to 2,384 results for: PRK

1. ERK1C82C202

   Plasmid

   #164650

   Purpose

   Bacterial expression plasmid for His6-ERK1C82C202

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   ERK1C82C202 (MAPK1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   K71R/C144S/C178S/C183S/T202C/C271S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. CRaf-IRES-pErk reporter

   Plasmid

   #226170

   Purpose

   Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)
   

   Use

   Tags

   3xFlag-tdMCP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. sh2g-ZMYND8-human

   Plasmid

   #201406

   Purpose

   Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

   Insert

   ZMYND8 (ZMYND8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-Nix-S212D

   Plasmid

   #197515

   Purpose

   Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

   Depositor

   Joseph Gordon

   Article

   da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

   Insert

   BCL2 interacting protein 3 like (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Serine 212 changed to aspartic acid

   Promoter

   CMV

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-Nix-S212A

   Plasmid

   #197514

   Purpose

   Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)

   Depositor

   Joseph Gordon

   Article

   da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

   Insert

   BCL2 interacting protein 3 like (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Serine 212 changed to alanine

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer-myc-ERK2-R67S/S153D

   Plasmid

   #213597

   Purpose

   Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R67S, S153D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_MAPK1_WT

   Plasmid

   #82145

   Purpose

   Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-MAPK1

   Plasmid

   #116760

   Purpose

   Lentiviral expression of MAPK1

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-MAPK1-E322K

    Plasmid

    #116432

    Purpose

    Lentiviral expression of MAPK1 E322K

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E322K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-MAPK14

    Plasmid

    #23865

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pWZL Neo Myr Flag MAPK14

    Plasmid

    #20524

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pInducer-myc-ERK2-R67S/S153D/D321N

    Plasmid

    #213598

    Purpose

    Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R67S, S153D, D321N

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pInducer-myc-ERK2-R67S/S153D/Y263A

    Plasmid

    #213599

    Purpose

    Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R67S, S153D, Y263A

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-MAPK1

    Plasmid

    #23498

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    missing first codon

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. Rheb WT

    Plasmid

    #24941

    Depositor

    Kunliang Guan

    Insert

    Rheb
    

    Use

    N/a

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Flag-WASP

    Plasmid

    #47432

    Depositor

    Peter McPherson

    Insert

    WASP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pPMS-2008

    Plasmid

    #232288

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Type

    Empty backbone

    Use

    Tags

    8X His-Avi-1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPMS-2272

    Plasmid

    #232292

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Insert

    Alpha bungarotoxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-CREST (C89)

    Plasmid

    #22961

    Depositor

    Anirvan Ghosh

    Insert

    CREST (Ss18l1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2010

    Availability

    Academic Institutions and Nonprofits only