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We narrowed to 16,014 results for: grna

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Showing: 1161 - 1180 of 16014 results

  2. pORANGE GFP-Camk2a KI

    Plasmid
    #131484
    Purpose
    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)
    Depositor
    Harold MacGillavry
    Publication
    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
    Expression
    Mammalian
    Promoter
    U6 and Cbh
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131484-plasmid-map-sequence-id-257183

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  3. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid
    #75409
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [D1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75409-plasmid-map-sequence-id-211946

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  7. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  9. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid
    #196086
    Purpose
    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting lncRNA NORAD
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196086-plasmid-map-sequence-id-388997

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  10. pUC-tRNAGln-BbsI-sU6-Kan

    Plasmid
    #213016
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)
    Depositor
    Stephanie Roessler
    Insert
    tRNAGln promoter, sU6 promoter, gRNA scaffolds
    Promoter
    tRNAGln promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213016-plasmid-map-sequence-id-423155

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  19. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55200-plasmid-map-sequence-id-85587

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Showing: 1161 - 1180 of 16014 results