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pcDNA3-iLMO2

Plasmid

#129474

Purpose

Mammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

Depositor

Robert Gross

Article

Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

Insert

iLMO2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

pCMV and T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc180

Plasmid

#161942

Purpose

Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc226

Plasmid

#161944

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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mPLD2-PX (62-192)

Plasmid

#119128

Purpose

Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mPLD2-PX (62-192)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST YFP Rab8 DN

Plasmid

#58451

Purpose

expression of drosophila YFP Rab8 dominant negative mutant

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab2 DN

Plasmid

#58445

Purpose

expression of drosophila YFP-Rab2 dominant negative mutant

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab2 (Rab2 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

S20N (dominant negative)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab2 CA

Plasmid

#58443

Purpose

expression of drosophila YFP-Rab2 constitutively active mutant

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab2 (Rab2 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

Q65L (constitutive active)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab14 DN

Plasmid

#37715

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab14 (Rab14 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S49N (dominant negative)

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
SNX26-PX (56-172)

Plasmid

#119105

Purpose

Bacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX26-PX (56-172)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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SNX28-PX (1-124)

Plasmid

#119107

Purpose

Bacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX28-PX (1-124)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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SNX34-PX (1-130)

Plasmid

#119113

Purpose

Bacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX34-PX (1-130)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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IRAS-PX (13-124)

Plasmid

#119118

Purpose

Bacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

IRAS-PX (13-124)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_SMU1:1-513

Plasmid

#210877

Purpose

Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SMU1:M1-P513 (SMU1 Human)

Use

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_MAGEA3:104-314

Plasmid

#210941

Purpose

Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MAGEA3:E104-E314 (MAGEA3 Human)

Use

Tags

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP15:1-370

Plasmid

#210937

Purpose

Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

UTP15:M1-H370 (UTP15 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TBL2:70-405

Plasmid

#210936

Purpose

Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TBL2:E70-H405 (TBL2 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR73:1-378

Plasmid

#210932

Purpose

Insect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR73:M1-R378 (WDR73 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR54:1-334

Plasmid

#210931

Purpose

Insect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR54:M1-V334 (WDR54 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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