162,982 results

1. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-U6-gRNA-CBh-mCherry

   Plasmid

   #91947

   Purpose

   Expression of empty gRNA cassette and mCherry

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB208

   Plasmid

   #164123

   Purpose

   Ptac driving PP7 repeats

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   YFP-PP7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA 1 GAPDH

   Plasmid

   #83808

   Purpose

   Express Sg-1 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-NLS-HA-TurboID

   Plasmid

   #215075

   Purpose

   Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. OmpX

   Plasmid

   #213136

   Purpose

   Expresses outer membrane protein X

   Depositor

   Sebastian Hiller

   Article

   Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

   Insert

   OmpX (24-171) (ompX E.coli (K12))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SypHer mt

   Plasmid

   #48251

   Purpose

   pH sensor, targeted to mitochondria

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Use

   Tags

   2 mitochondrial targeting sequences

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLdCN2

   Plasmid

   #125186

   Purpose

   Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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