We narrowed to 18,078 results for: comp

1. pSLIK-IDH2-R172K-FLAG

   Plasmid

   #66807

   Purpose

   Tet-inducible expression of mutant IDH2 in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   IDH2 (IDH2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-gfap

   Plasmid

   #75024

   Purpose

   Can be used to drive expression in zebrafish astrocytes.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   glial fibrillary acidic protein promoter (gfap Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   gfap

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189922

   Purpose

   AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SaCas9 D10A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK-IDH2-FLAG

   Plasmid

   #66806

   Purpose

   Tet-inducible expression of IDH2 in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   IDH2 (IDH2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYO1031

   Plasmid

   #235706

   Purpose

   Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

   Inserts

   Beclin 1 (BECN1 Human)
   UVRAG (UVRAG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-AR Q0

   Plasmid

   #86427

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the poly-glutamine expansion

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Parkin-IRES-zeo

   Plasmid

   #61727

   Purpose

   Expression of human Parkin fused to mCherry

   Depositor

   Stephen Tait, Douglas Green

   Article

   Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Str-KDEL_CD63-SBP-mCherry

   Plasmid

   #222326

   Purpose

   Synchronize the trafficking of CD63 from the ER.

   Depositor

   Franck Perez

   Article

   Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

   Insert

   Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-FAB(PX-LD)

   Plasmid

   #214422

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSD025A

    Plasmid

    #218128

    Purpose

    Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII-neg
    

    Use

    Tags

    scP22

    Expression

    Mutation

    W384A, Y386A

    Promoter

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-Hygro-EF1A-EGFP-PARP1

    Plasmid

    #176146

    Purpose

    EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Coff/Fon-iC++-EYFP

    Plasmid

    #137157

    Purpose

    Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171381

    Purpose

    Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13Y.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189924

    Purpose

    AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SaCas9 D10A, TadA ABE8e V106W

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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