We narrowed to 20,125 results for: Kin

1. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

   Plasmid

   #208964

   Purpose

   Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3_T7-mScarlet3-KRas6R

   Plasmid

   #225930

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-KRas6R
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 10_T7-SP-CD8tm-mScarlet3

   Plasmid

   #225937

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   SP-CD8tm-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCALPS-mAgo2-EGFP

   Plasmid

   #209935

   Purpose

   Lentiviral expression of mouse Argonaute-2 in mammalian cells

   Depositor

   Phillip Zamore

   Article

   Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiex PKC - Peptide 8 GFP RFP

   Plasmid

   #83564

   Purpose

   Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRAV8-1

   Plasmid

   #123375

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV8-1 (TRAV8-1 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CIB-VP16 (B1014)

   Plasmid

   #92036

   Purpose

   Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Tags

   Fusion of plant CIB1 (intact NLS) with VP16 activ…

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-Venus-MDGA2

   Plasmid

   #82544

   Purpose

   Overexpression vector for Venus-MDGA2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA2 (Mdga2 Rat)
   

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Promoter

   chicken beta actin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-P2A-CIAR_pLenti_CMV_Puro

   Plasmid

   #86503

   Purpose

   Lenti plasmid for generating CIAR expressing stable cell lines

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   GFP-P2A-CIAR (SOS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRE-TIGHT-Cx43-eYFP

    Plasmid

    #31807

    Depositor

    Robin Shaw

    Article

    Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

    Insert

    Cx43 (GJA1 Human)
    

    Tags

    eYFP

    Expression

    Mammalian

    Promoter

    P-TIGHT

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pTXB1-hMeCP2

    Plasmid

    #48091

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. kiCAP-AAV-M.Mus.2

    Plasmid

    #196682

    Purpose

    Rep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    REQQKLW insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1 shJUND puro

    Plasmid

    #58698

    Purpose

    Lentiviral shRNA vector for knockdown of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shJUND (JUND Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3 βarr1 S412D Flag

    Plasmid

    #42196

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    β-arrestin 1 S412D (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S412D

    Promoter

    CMV

    Available Since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pX459-HypaCas9-ANLN_sgRNA

    Plasmid

    #183874

    Purpose

    pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Fyn-SH3_5WT_S25C

    Plasmid

    #118824

    Purpose

    Bacterial expression of the SH3 domain of human Fyn

    Depositor

    Nicolas Fawzi

    Article

    Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

    Insert

    FYN (FYN Human)
    

    Tags

    6-His-TEV tag

    Expression

    Bacterial

    Mutation

    S25C; See depositor comments below.

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pClneoMyc human MST2

    Plasmid

    #37022

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MST2 (STK3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

20. GFP-FKBP-TPD54

    Plasmid

    #172454

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    GFP-FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge