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pAAV-CaMKIIa-HA-eLACCO2.1-NGR

Plasmid

#207989

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yuki Kambe

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Promoter

CaMKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_Rab11a

Plasmid

#65705

Purpose

Expresses YNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCardinal-Tubulin-C-18

Plasmid

#56176

Purpose

Localization: Microtubules, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tubulin (TUBA1B Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1 His-V5 Myc dMBIV

Plasmid

#176055

Purpose

Expresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 304-324

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIb

Plasmid

#176054

Purpose

Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 259-270

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIa

Plasmid

#176053

Purpose

Expresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 188-199

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBII

Plasmid

#176052

Purpose

Expresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 128-143

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBI

Plasmid

#176051

Purpose

Expresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 44-63

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMB0

Plasmid

#176050

Purpose

Expresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 16-33

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-730pUC19-tNGFR-P2A-Coronin1A

Plasmid

#186110

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CORONIN1A

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-mCherry

Plasmid

#65283

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-PDHA1-N-10

Plasmid

#56172

Purpose

Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

PDHA1 (PDHA1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
H2B-mAvicFP1

Plasmid

#129511

Purpose

Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

H2B-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 fused to the C terminus of human histone…

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(R438TAG)

Plasmid

#182662

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position R438 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

R438TAG

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA1

Plasmid

#138190

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA2

Plasmid

#138191

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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