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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti GW-mCit-PA

Plasmid

#113457

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

mCitrine-ProteinA

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-Erk2 donor

Plasmid

#113104

Purpose

Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

N terminal Erk2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_EZRI

Plasmid

#111665

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

EZRI (EZR Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-CFP-FRBx5

Plasmid

#103780

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMThy1.1_hDDX5_FL_D248N

Plasmid

#88874

Purpose

constitutive expression of DDX5 D248N in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D248N

Promoter

MSCV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA2

Plasmid

#73982

Purpose

Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

EGFP-Otx2-shRNA2

Use

RNAi

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-Rap2A-3xFLAG

Plasmid

#87973

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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289 RFP/CFP

Plasmid

#83321

Purpose

fluorescent signal joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHis6-hDF

Plasmid

#72585

Purpose

human dysferlin full length tagged with His

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-B-161;+80pCalb2

Plasmid

#66745

Purpose

luciferase reporter for Calb2 promoter (-161to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Paxillin-22

Plasmid

#57796

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK0

Plasmid

#45047

Purpose

FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-FLAG-Neuroserpin

Plasmid

#58261

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pEntry Tshld-GFP

Plasmid

#53211

Purpose

pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld-GFP

Tags

EGFP, Flag, and HA

Promoter

none

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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