We narrowed to 11,396 results for: ENA

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pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1K91A

Plasmid

#133248

Purpose

Express FLAG-tagged MCART1K91A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, K9…

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-GFP-PGK-Puro

Plasmid

#110868

Purpose

Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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FXYD2-luc

Plasmid

#31056

Depositor

Gerhart Ryffel

Article

Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

Insert

FXYD2 (FXYD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

FXYD2 promoter from -2382 to -1

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1-GFP

Plasmid

#111657

Purpose

C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1-GFP (Bicdl1 Mouse)

Tags

8xHis-tag, GFP, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS423-mitoT

Plasmid

#174525

Purpose

Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

Yeast mitochondrial intermembrane tether

Use

Synthetic Biology

Tags

eGFP

Expression

Yeast

Promoter

MET25

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b(+)-ATI_QconCAT_1

Plasmid

#163957

Purpose

Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

Depositor

Stefan Tenzer

Article

Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

Insert

ATI QconCAT

Tags

polyhistidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMTB2-cytoBirA-2A-mCherry_Ras

Plasmid

#80066

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a-ncOGT-TPR

Plasmid

#190822

Purpose

For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

6x His

Expression

Bacterial

Mutation

C467X. Also codon optimized for expression in E.c…

Promoter

T7

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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