We narrowed to 8,594 results for: Set

1. ACY

   Plasmid

   #12396

   Depositor

   David Baker

   Article

   Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

   Insert

   ACY
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RosettaDesign was used to design sequences. Align…

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

2. TEN

   Plasmid

   #12498

   Depositor

   David Baker

   Article

   Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

   Insert

   TEN
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RosettaDesign was used to design sequences. Align…

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-U6-gRNA-CBh-mCherry

   Plasmid

   #91947

   Purpose

   Expression of empty gRNA cassette and mCherry

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   mCherry
   

   Use

   AAV

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-DRGFP

   Plasmid

   #113193

   Purpose

   DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   DR-GFP
   

   Use

   Donor construct with t2a-hygromycin for gene trap…

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH136

   Plasmid

   #47554

   Purpose

   pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

   Insert

   URA3
   

   Use

   Pcr template

   Promoter

   Native URA3 promoter, -243 to -1.

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide-BC-EF1a-Puro

   Plasmid

   #127170

   Purpose

   PCR template for EF1a-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVTHM

   Plasmid

   #12247

   Purpose

   2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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