179,359 results

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Publication

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Promoter

human Synapsin

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX037

Plasmid

#167156

Purpose

MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

Depositor

Karen Sarkisyan

Publication

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

caffeic acid cycle genes

Expression

Plant

Promoter

pNOS, p35s

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Plasmid L5 attB::Pleft* mWasabi

Plasmid

#169409

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

Depositor

Clifton Barry III

Publication

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mWasabi

Use

Unspecified

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Publication

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Publication

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

SFFV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Publication

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPGK-T7/2-CD44s (fl)

Plasmid

#137812

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Publication

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length, I186T and I253V on CD44

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Publication

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HSP70 (HSPA1A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GZnP3 in pcDNA3.1+

Plasmid

#161738

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Publication

Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GZnP3

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSMAL

Plasmid

#161785

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV6-Neo-ISG15

Plasmid

#80404

Purpose

overexpression of ISG15

Depositor

Isidoro Martinez

Publication

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ISG15 (ISG15 Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

Plasmid

#160540

Purpose

Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

Depositor

Kenneth Johnson

Publication

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Inserts

SARS-CoV-2 NSP7 (ORF1ab Synthetic)
SARS-CoV-2 NSP8 (ORF1ab Synthetic)
SARS-CoV-2 NSP12 (ORF1ab Synthetic)

Expression

Bacterial

Available since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

Plasmid

#160369

Purpose

Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

Depositor

David Sabatini

Publication

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-3xHA

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

CFP

Expression

Mammalian

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-S5E2-Gq-P2A-dTomato

Plasmid

#135635

Purpose

AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1, AAV9, and AAV PHP.eB

Insert

Gq-P2A-dTomato

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Publication

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA1h

Plasmid

#140557

Purpose

Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1h (high affinity)

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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