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pcDNA 3.1(-) rat C/EBP beta (LAP)

Plasmid

#12558

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pAJE3-E7

Plasmid

#214359

Purpose

Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

Depositor

Ryan Mehl

Article

Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

Inserts

2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin

Expression

Bacterial

Promoter

AmpR and lpp

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-GFP

Plasmid

#100726

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA-mCherry

Plasmid

#99878

Purpose

Expression of GFP-AURKA-mCherry in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Mammalian

Promoter

AURKA minimal

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDule-Tet2.0

Plasmid

#85496

Purpose

Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

Insert

Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Tags

None

Expression

Bacterial

Mutation

Y32G L65Q F108S Q109D D158S L162N

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV14/p14ARF

Plasmid

#78760

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Ascl2

Plasmid

#71151

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ascl2 (Ascl2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB4-SV40-GFP (B4G)

Plasmid

#65444

Purpose

Expression of human EPHB4 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB4 (EPHB4 Human)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBa-flag-BicD2 594-FKBP

Plasmid

#64206

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-C (880-1475)

Plasmid

#64317

Purpose

GFP-tagged mammalian expression of C-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal subunit (880-1475)

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pB2-neo-TET

Plasmid

#51287

Purpose

expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

sine B2

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

enhancer from 7SL gene

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pMCS.71/2272

Plasmid

#22732

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-MCS-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE containi…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a MICA*009-IRES-ZsGreen

Plasmid

#114007

Purpose

Induces expression of human MICA allele 009 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICA*009 (MICA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-AID*-9myc

Plasmid

#99524

Purpose

C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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