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pRDA355_sgCiPELO #2

Plasmid

#229020

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-COV E

Plasmid

#165123

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV E (E codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF9b

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2Cx32WTEGFP

Plasmid

#78150

Purpose

Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

Depositor

Mario Bortolozzi

Article

Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

Insert

Connexin 32 (GJB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Bmi1

Plasmid

#131603

Purpose

expression of mouse Bmi1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Bmi1 (Bmi1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216

Plasmid

#167354

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_174_neoR

Plasmid

#228935

Purpose

Lentiviral expression of EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ETV6

Plasmid

#131594

Purpose

doxycycline-inducible expression of human ETV6 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

ETV6 (ETV6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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