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pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Tags

none

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/CSH3* (P833L) uGFP

Plasmid

#14566

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CSH3* (P833L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
RAB14 3'UTR D1

Plasmid

#61490

Purpose

luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-Purg1-GST

Plasmid

#39352

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GST and kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (S44D)

Plasmid

#24235

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Article

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSP64R1 SHP2 E76A

Plasmid

#8327

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

E76A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
PX458_HLF_2

Plasmid

#86302

Purpose

Encodes gRNA for 3' target of human HLF

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HLF (HLF Human)

Use

CRISPR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HLF

Plasmid

#86262

Purpose

Donor vector for 3' FLAG tag of human HLF

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HLF homology arms (HLF Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HLF_1

Plasmid

#86301

Purpose

Encodes gRNA for 3' target of human HLF

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HLF (HLF Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Rhoc-wt_3'UTR

Plasmid

#31884

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc wild-type 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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