We narrowed to 13,846 results for: CRISPR-Cas9
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Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E7
Plasmid#213470PurposeGateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cdd-32aa linker-z…TagsNoExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E8
Plasmid#213471PurposeGateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cdd_v106w-32aa li…TagsNoExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME463
Plasmid#238103PurposeExpressed ME-ABE ABE7.10-HK with SpRYCas9DepositorInsertTadA*7.10(Y123H/N157K)-m_SpRYnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include Y123H, N157K; SpRY-nCa…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pME234
Plasmid#238104PurposeExpresses ME-ABE ABE7.10-HK with SpCas9 (NGDepositorInsertTadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include Y123H, N157K; NG-nCas9…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 V82G ABEMax Strategy 3
Plasmid#135363PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminusDepositorInsert1029 V82G ABEMax
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN
Plasmid#85754PurposeExpresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-dCas9
UseCRISPRTagsCsy4 and FokI fusion via 25 amino acid (GGGGS)5 l…ExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME464
Plasmid#238068PurposeExpressed ME-ABE ABE7.10-HRHSK with SpRYCas9DepositorInsertTadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include L36H, L51R, Y123H, C14…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N1
Plasmid#173998PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10AAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O1
Plasmid#173999PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10AAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-Ds
Plasmid#119069PurposeTo clone sgRNA spacer sequence for microinjections in zebrafishDepositorInsertU6a:sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds
Plasmid#119070PurposeConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled1-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds
Plasmid#119071PurposeConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds
Plasmid#119072PurposeConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled3-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds
Plasmid#119073PurposeConstruct with scrambled-4 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled4-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23A
Plasmid#91138PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterAtUbi10Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0601
Plasmid#91016PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRMutationD10APromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only