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178,384 results

Showing: 5981 - 5990 of 178384 results

  1. pKC-4.06

    Plasmid
    #112084
    Purpose
    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)
    Depositor
    James Inglese
    Publication
    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Human, Photinus pyralis, Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112084-plasmid-map-sequence-id-214510

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  4. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid
    #104487
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7s
    Use
    AAV
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104487-plasmid-map-sequence-id-241275

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  7. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100902-plasmid-map-sequence-id-195323

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Showing: 5981 - 5990 of 178384 results