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pnEA-NpH-DmeIF4E_69-248-I96AI112A_Z

Plasmid

#148173

Purpose

Bacterial Expression of DmeIF4E_69-248-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E_69-248-I96AI112A

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6C_1-1207_M

Plasmid

#146944

Purpose

Mammalian Expression of HsTNRC6C_1-1207

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1-1207 (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-siRNAres_I

Plasmid

#146602

Purpose

Mammalian Expression of HsTNRC6C-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C-siRNAres (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_1-1254_H

Plasmid

#146511

Purpose

Mammalian Expression of HsTNRC6C_1-1254

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1-1254 (TNRC6C Human)

Expression

Mammalian

Mutation

two silent mutations C2349T and A2547C compared t…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delAGOhook_F

Plasmid

#146307

Purpose

Mammalian Expression of HsTNRC6C-delAGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delAGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

1 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI_F

Plasmid

#146308

Purpose

Mammalian Expression of HsTNRC6C-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-AGOhook_F

Plasmid

#146309

Purpose

Mammalian Expression of HsTNRC6C-delMI-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-AGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

1 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMII_F

Plasmid

#146310

Purpose

Mammalian Expression of HsTNRC6C-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMII (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII_F

Plasmid

#146311

Purpose

Mammalian Expression of HsTNRC6C-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-MII (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA_D

Plasmid

#146140

Purpose

Mammalian Expression of tubcDNA

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC62_D

Plasmid

#146142

Purpose

Mammalian Expression of tubcDNA-PTC62

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC62

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_L

Plasmid

#146865

Purpose

Mammalian Expression of HsTNRC6C

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6C (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4E_E

Plasmid

#146188

Purpose

Insect Expression of DmeIF4E

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmeIF4E

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf

Plasmid

#182000

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_SKL

Plasmid

#182007

Purpose

CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf_SKL

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvH-DmeIF4E-I96AI112A_Z

Plasmid

#148196

Purpose

Bacterial Expression of DmeIF4E-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E-I96AI112A

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEK-NvSHN-DmeF4E-I96AI112A_Z

Plasmid

#148197

Purpose

Bacterial Expression of DmeIF4E-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E-I96AI112A

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge