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Plasmid#101157PurposeLentiviral expression of SmyD1 and copGFPDepositorInsertSmyD1 (Smyd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-C600
Plasmid#92324PurposeC-term of HCF-1 proteinDepositorInsertHCF-1 C-term 1436-2035 (HCFC1 Human)
UseTagsC-myc (EQKLISEED) and HAExpressionMammalianMutationAA 1436-2035PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1 WT
Plasmid#114049PurposeExpression of exogenous LacI-GFP-HEC1 WTDepositorInsertGFP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationWT HEC1 sequencePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFM038
Plasmid#40141DepositorInsertpar-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + 7SA; base pairs 6-523 of par-2 were re…Promoterpie-1Available sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM041
Plasmid#40144DepositorInsertpar-2 RNAi resistant (S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationS241A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM039
Plasmid#40142DepositorInsertpar-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + S241A; base pairs 6-523 of par-2 were …Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM036
Plasmid#40139DepositorInsertpar-2 RNAi resistant (K162A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationK162A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pT7-gfap-sgRNA
Plasmid#65566Purposein vitro trancription of sgRNA targeting the zebrafish gfap locusDepositorInsertgfap sgRNA (gfap Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011Δ382-450
Plasmid#92322PurposeN-term of HCF-1 protein without amino acids 382-450DepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-1011 with internal deletion Δ382-450PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N450
Plasmid#92321PurposeN-term 1-450 of HCF-1 proteinDepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-450. Note that a SRRASGTYGRV amino-terminal …PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2591
Plasmid#144211PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF354C (ZNF354C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1-d207
Plasmid#114050PurposeExpression of exogenous LacI-GFP-HEC1 delta 207DepositorInsertGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d207
Plasmid#114051PurposeExpression of exogenous eGFP HEC1 delta 207DepositorInserteGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only