We narrowed to 299 results for: IPTG

1. pMM731

   Plasmid

   #68896

   Purpose

   IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM705

   Plasmid

   #68892

   Purpose

   IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ291_WT_med

   Plasmid

   #128856

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM763

   Plasmid

   #68900

   Purpose

   IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   G1104E

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/mlcR25-mEGFP

   Plasmid

   #154295

   Purpose

   Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Insert

   25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJUMP19-Ptac_P

   Plasmid

   #127004

   Purpose

   Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part Ptac_P
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JC101

   Plasmid

   #104952

   Purpose

   Methanol induing RFP, estrogen inducing GFP, and IPTG inducing CFP

   Depositor

   Timothy Lu

   Article

   Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

   Insert

   GFP, mCherry, and CFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOGG202

   Plasmid

   #115503

   Purpose

   Clone of a level 1 unit which encodes IPTG-inducible sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ291_WT_low

    Plasmid

    #128855

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM725

    Plasmid

    #68895

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMM750

    Plasmid

    #68899

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JC031

    Plasmid

    #104953

    Purpose

    Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing Interferon

    Depositor

    Timothy Lu

    Article

    Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

    Insert

    hGH, Interferon and G-CSF
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM732

    Plasmid

    #68897

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKDC.109

    Plasmid

    #227189

    Purpose

    T7 IPTG-driven Cas9

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    SpyCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKDC.118

    Plasmid

    #227188

    Purpose

    T7 IPTG-driven Cas13a

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    LbuCas13a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MDH1_KW

    Plasmid

    #224145

    Purpose

    IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

    Depositor

    Katherine Walstrom

    Article

    Thomas et al Biochim Biophys Acta Proteins Proteom. 2

    Insert

    MDH-1 (F46E10.10 Nematode)
    

    Use

    Tags

    Ala-intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC17V5a

    Plasmid

    #197413

    Purpose

    IPTG-inducible two-plasmid system bearing CP25 > lacO-GFP, specR, RSF1010 replicon (1/2)

    Depositor

    Yolanda Schaerli

    Article

    Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

    Insert

    lacO-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC17V5b

    Plasmid

    #197414

    Purpose

    IPTG-inducible two-plasmid system bearing CP25 > lacI, ampR, pTF-FC2 replicon (2/2)

    Depositor

    Yolanda Schaerli

    Article

    Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

    Insert

    lacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbA5k-AcrAB-AsLOV2*(543)

    Plasmid

    #210863

    Purpose

    IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Inserts

    acrA
    acrB
    

    Use

    Synthetic Biology

    Tags

    AsLOV2*(543)

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBW016

    Plasmid

    #131820

    Purpose

    pET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTXB1-Tn5

    Plasmid

    #60240

    Purpose

    IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Rickard Sandberg

    Article

    Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

    Insert

    tnpA from pBam1
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7 promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEutRwc

    Plasmid

    #216334

    Purpose

    An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

    Depositor

    Mark Styczynski

    Article

    McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

    Inserts

    EutR
    Superfolder green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pTD-NStrepHis

    Plasmid

    #45936

    Purpose

    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET11a-link-NGFP

    Plasmid

    #52732

    Purpose

    IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-NGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p5T7-LYCipi-ggpps

    Plasmid

    #122017

    Purpose

    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Insert

    GGPP synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized, truncated first 98 amino acids, …

    Promoter

    T7

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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