We narrowed to 2,422 results for: NIG

1. pCOLA-Spec-yibDp-Erv1p

   Plasmid

   #212094

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   Sulfydryl oxidase (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. STAgR_tdTomato

   Plasmid

   #102993

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCOLA-Gent-yibDp-Erv1p

   Plasmid

   #202483

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBO.ACT005

   Plasmid

   #182715

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STAGR_SAMScaffold_mU6

   Plasmid

   #102845

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_mU6
   

   Use

   Pcr template for stagr inserts

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBSSK p85 alpha (delta SH3)

   Plasmid

   #13430

   Depositor

   Ronald Kahn

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

   Insert

   p85 alpha (delta SH3) (PIK3R1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   SH3 domain replaced by flag tag.

   Promoter

   Available since

   Nov. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

8. STAGR_SAMScaffold_hH1

   Plasmid

   #102847

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_hH1
   

   Use

   Pcr template for stagr inserts

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pyc128m

   Plasmid

   #158778

   Purpose

   soluble GCaMP expression

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBSSK p85 alpha (delta BH)

    Plasmid

    #13431

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta BH) (PIK3R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    BH domain replaced by flag tag.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

11. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    H7SK

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-GcACR_457-EYFP

    Plasmid

    #103137

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_457
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACEBac1_Strep-Psc-BICD2(711-800)_L786A

    Plasmid

    #228838

    Purpose

    Insect cell expression of the mutant C-terminus of BICD2

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800, with L786A

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACEBac1_Strep-Psc-BICD2(711-800)

    Plasmid

    #228833

    Purpose

    Insect cell expression of CC3 domain of BICD2 (aa 711-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACEBac1_Strep-Psc-BICD2(342-800)

    Plasmid

    #228832

    Purpose

    Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 342-800

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACEBac1_Strep-Psc-BICD2(711-800)_L742A

    Plasmid

    #228839

    Purpose

    Insect cell expression of the mutant C-terminus of BICD2

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800, with L742A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMART-HCKan-yibDp-VHH-GFPenhancer

    Plasmid

    #202468

    Purpose

    Expresses the nanobody GFP enhancer after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP enhancer
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMART-HCKan-yibDp-VHH-GFPminimizer

    Plasmid

    #202469

    Purpose

    Expresses the nanobody GFP minimizer after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSMART-HCKan-yibDp-roGFP2

    Plasmid

    #202462

    Purpose

    Expresses redox sensitive GFP (roGFP) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTSAR3.6t

    Plasmid

    #210258

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mScarlet
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTSAR3.7t

    Plasmid

    #210259

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    e2Crimson
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTSAR3.3t

    Plasmid

    #210255

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    DsRed
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPBO.ACT002

    Plasmid

    #182712

    Purpose

    aTc inducible dCasRx-IF1

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 1

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-G1ACR_203-EYFP

    Plasmid

    #103143

    Purpose

    Anion channelrhodopsin from Geminigera sp. (Caron Lab Isolate) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin G1ACR_203
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-GcACR_145-EYFP

    Plasmid

    #103138

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_145
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-GcACR_439-EYFP

    Plasmid

    #103142

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_439
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag hHMGB1

    Plasmid

    #31609

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCOLA-Gent-EM7-Erv1p-DsbC

    Plasmid

    #202485

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Tet-O-FUW-FoxG1

    Plasmid

    #35547

    Depositor

    Marius Wernig

    Article

    Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    FoxG1 (Foxg1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 Flag mHMGB2

    Plasmid

    #31610

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB2 (Hmgb2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pyc111m

    Plasmid

    #158779

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBSSK p85 alpha (delta iSH2)

    Plasmid

    #13432

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta iSH2) (PIK3R1 Human)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Dominant negative p85 alpha. The p110 alpha bindi…

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pAS18

    Plasmid

    #158787

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Ptag-168::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBSSK p85 alpha (delta SH3, iSH2)

    Plasmid

    #13433

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3, iSH2) (PIK3R1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Flag tags replacing both the SH3 and iSH2 domains.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pAS19

    Plasmid

    #158788

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    psra-6::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAS16

    Plasmid

    #158786

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Pntc-1::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ptia1l-hsp-GFP

    Plasmid

    #177837

    Purpose

    Donor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9

    Depositor

    David Kingsley

    Article

    Wucherpfennig et al Nat Ecol Evol. 2022 Sep 1. pii: 10.1038/

    Insert

    tia1l-sgRNA target site-hsp70-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    hsp70

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHJ4

    Plasmid

    #158785

    Purpose

    Pan-neuronal nuclear-localized GCaMP expression in C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Prab-3::nls::GCaMP6m
    

    Use

    Tags

    NLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. TetO-Ascl1-puro

    Plasmid

    #97329

    Purpose

    Generating lentiviruses to express Ascl1 and puromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TET-ON

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. PiggyBac-TetO-Ascl1-neomycin

    Plasmid

    #176482

    Purpose

    TetO-Ascl1 in a PiggyBac vector with neomycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pTet-O-Nd1-puro

    Plasmid

    #52052

    Purpose

    Expresses Nd1 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Neurod1 (Neurod1 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

    Plasmid

    #184380

    Purpose

    The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJV126

    Plasmid

    #124250

    Purpose

    In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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