We narrowed to 145 results for: B SUBTILIS¨

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pDR111_mannose_Bgal NLS

Plasmid

#188398

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBS2E

Plasmid

#55169

Purpose

Empty vector, integration at lacA, ampr, mlsr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pDRF1-GW yAT1.03

Plasmid

#132781

Purpose

FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03 ymTq2-tdTomato

Expression

Yeast

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHT-6xR.ads-PxylA-DI

Plasmid

#47386

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

ads
dxs
idi

Tags

6xR and His

Expression

Bacterial

Promoter

Pgrac and PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pWH-DI

Plasmid

#47385

Purpose

Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

dxs
idi

Expression

Bacterial

Promoter

PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only
pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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PliaG

Plasmid

#55174

Purpose

Constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

LiaG promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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PliaI

Plasmid

#55177

Purpose

Bacitracin-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

liaI promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

liaI

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLIC.B4.BSU37660

Plasmid

#11535

Depositor

Sung-Hou Kim

Article

Xu et al J Struct Funct Genomics. 2005 Dec;6(4):2

Insert

phosphotransacetylase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-B-mSpyCatcher-C

Plasmid

#87374

Purpose

An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

mSpyCatcher

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yAT1.03R122KR126K

Plasmid

#132782

Purpose

Non-binding version of the FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03R122KR126K ymTq2-tdTomato

Expression

Yeast

Mutation

R122K, R126K

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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