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Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL32 (in cHNL135)
Plasmid#24387DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHC_YEAST
Plasmid#204232PurposeBacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC_YEAST (MDH2 Budding Yeast)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHC1_ARATH
Plasmid#204237PurposeBacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC1_ARATH (AT1G04410 Mustard Weed)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDH_PFALCI
Plasmid#204235PurposeBacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK142
Plasmid#73317PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p, and c-mycExpressionYeastMutationsynthetic gene. Encodes a glycine-204 to serine m…PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_HALMA
Plasmid#204241PurposeBacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_HALMA (mdh Haloarcula marismortui)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Clim Act MCH (CAM)
Plasmid#104443PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim EF1 MCH (CEM)
Plasmid#104444PurposeExpresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoterEF1Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Tub MCH (CTM)
Plasmid#104445PurposeExpresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromotertubulin promoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-MokolaG
Plasmid#15811DepositorInsertMokola virus glycoprotein (G) gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAH01
Plasmid#128162PurposeIncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-EcoEnv
Plasmid#15802DepositorInsertMLV env (ecotropic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-EV
Plasmid#202466PurposeEmpty vector of pSMART-HCKanDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ329: pCMV-MmeFz2
Plasmid#205266PurposeHuman expression vector for human codon optimized MmeFz2DepositorInsertMmeFz2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRL488
Plasmid#70263Purposedesigned to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.DepositorTypeEmpty backboneUseLuciferase; Cyanobacteria expressionTagsluxABExpressionMutationPromoternoneAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EV
Plasmid#202491PurposepCOLA-Gent Empty VectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only