Addgene

Showing: 101 - 120 of 40272 results

Results per page:

Sort results by:

pVITRO1-SS-257

Plasmid

#68774

Purpose

C Trans V2

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

C-terminal Sensor 1 V2

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Use

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-345

Plasmid

#68785

Purpose

R->A Sensor pair 1 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-247

Plasmid

#68761

Purpose

ZF9-TF-V2 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9-TF-V2
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Citroff1

Plasmid

#134304

Purpose

Mammalian expression of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-Citron1

Plasmid

#134300

Purpose

Bacterial expression of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Use

Tags

6-His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pN3-Control

Plasmid

#24544

Depositor

Guntram Suske

Article

Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
U6.3>Control.gRNA.f+e

Plasmid

#99140

Purpose

Control gRNA under the regulation of an optimized chicken specific U6 promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

Control gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Chicken U6.3

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2_claudink:Cas9green; control_gRNA

Plasmid

#199336

Purpose

pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
control sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Voltron-ST

Plasmid

#119034

Purpose

Express soma-localized Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-CitroffRH

Plasmid

#158546

Purpose

Mammalian expression of nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-dV-IgE/κ

Plasmid

#61878

Purpose

Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin Epsilon constant region
Human immunoglobulin kappa constant region

Use

Tags

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-EBOV-GP

Plasmid

#215692

Purpose

Produces the Ebola virus glycoprotein

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

Insert

EBOV-GP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDule-3-nitroTyrosine (5B)

Plasmid

#85498

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

None

Expression

Bacterial

Mutation

Y32H H70C D158S I159A L162R

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-dV-IgE/λ

Plasmid

#61879

Purpose

Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin Epsilon constant region
Human immunoglobulin lambda constant region

Use

Tags

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge