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pCAGGS-GCaMP2

Plasmid

#18926

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2

Tags

His

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3

Plasmid

#172921

Purpose

For enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3

Depositor

GENIE Project

Has Service

AAV1 and AAV9

Insert

axon-jGCaMP8s-P2A-mRuby3

Use

AAV

Tags

GAP43 palmitoylation domain and P2A-mRuby3

Expression

Mammalian

Promoter

hSynapsin1

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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GCaMP-AMPK-SPARK

Plasmid

#226257

Purpose

Dual reporter of Ca2+ levels and AMPK activity

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

GCaMP-AMPK-SPARK

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-GCaMP2-actin

Plasmid

#18928

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2-Actin (ACTB Chicken)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pZac2.1 gfaABC1D-Cyto-GCaMP3

Plasmid

#44331

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

GCaMP3

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Lck-GCaMP3

Plasmid

#44330

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

Lck-GCaMP3

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_GCaMP6s

Plasmid

#164589

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

Orai1-GCaMP6f (ORAI1 Synthetic, Human)

Tags

GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-GCaMP6s

Plasmid

#171156

Purpose

Encodes GCaMP6s

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

GCaMP6s

Expression

Mammalian

Available Since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS008: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163045

Purpose

GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6f

Plasmid

#80317

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6f

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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