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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

I179N

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Endogenous T/brachyury

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P200H

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W

Plasmid

#126522

Purpose

Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.

Depositor

Rusty Lansford

Article

Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. d

Insert

H2B-CeruleanFP-2A-Dendra2FP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hUbC

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Human T (BRACHYURY) sgRNA

Plasmid

#59726

Purpose

Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hT sgRNA (TBXT Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
HF-PX459 (V2)

Plasmid

#118632

Purpose

Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

Depositor

Mike McGrew

Article

Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

Insert

hSpCas9-HF1-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Asparagine 497 to Alanine, Arginine 661 to Alanin…

Promoter

Cbh

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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ROSA26(MultiFPsΔPuro)

Plasmid

#140759

Purpose

Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Inserts

puromycin-N-acetyltransferase
Venus
mCherry
mCerulean

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

CAGGS

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
FOR human NANOS3 TALEN

Plasmid

#59722

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
REV human NANOS3 TALEN

Plasmid

#59723

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pX330A-Yap1-E1-#1

Plasmid

#171517

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E2-#1

Plasmid

#171518

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge