We narrowed to 198 results for: rosa26

1. pGEM-floxedEGFP-TRI2A

   Plasmid

   #74669

   Purpose

   floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus

   Depositor

   Roberto Giovannoni

   Insert

   floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBigT

   Plasmid

   #21270

   Purpose

   Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pARv-RFP

   Plasmid

   #60021

   Purpose

   pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRUSH

   Plasmid

   #54480

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRUSH

   Plasmid

   #54479

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-Cas9-162A

   Plasmid

   #84918

   Purpose

   for in vitro transcription of Cas9 with polyA tail encoded

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Cas9
   

   Use

   Tags

   Flag, NLS, and polyA tail

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCS.71/2272.Hygro

    Plasmid

    #22731

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-FRT-PGK-hygro-FRT-Lox2272
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    This is a basal exchange vector for RMCE. The vec…

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCS.71/2272

    Plasmid

    #22732

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-MCS-Lox2272
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    This is a basal exchange vector for RMCE containi…

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG-2.1T-neo-sgROSA

    Plasmid

    #105587

    Purpose

    lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting rosa26 locus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCS.DT-A

    Plasmid

    #22730

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    DT-A
    

    Use

    Bac recombineering

    Tags

    Expression

    Mutation

    This is a gap-repair vector designed for retrievi…

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSwith-tdTomato

    Plasmid

    #60953

    Purpose

    Cre recombinase-mediated cassette exchange

    Depositor

    Liangxue Lai

    Article

    Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCA.66/2272

    Plasmid

    #22733

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox66-pU(delta)TK-EM7Neo-Lox2272
    

    Use

    Cre/Lox; Bac recombineering

    Tags

    Expression

    Mutation

    This vector was designed for assembling gene targ…

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ai65(RCFL-tdT) targeting vector

    Plasmid

    #61577

    Purpose

    Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    pCAG-FSF-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ai66(RCRL-tdT) targeting vector

    Plasmid

    #61578

    Purpose

    Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-RSR-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6-sgR26-eCas9-T2A-BlastR

    Plasmid

    #172493

    Purpose

    For CRISPR-assisted HDR, Rosa26 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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