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pAN-PBAD-sgRNA-A4NT

Plasmid

#62261

Purpose

expression of A4NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3T

Plasmid

#62260

Purpose

expression of A3T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3NT

Plasmid

#62259

Purpose

expression of A3NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y91F

Plasmid

#47093

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y91F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L

Plasmid

#25762

Purpose

Entry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Use

Tags

myc-tag and twhh promoter (D rerio)

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-B/c

Plasmid

#227962

Purpose

Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acid Research 2025

Insert

B/c

Use

Tags

Expression

Mutation

Promoter

None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Expression

Mutation

Promoter

TRE3GV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Gag

Plasmid

#221325

Purpose

Dox-inducible expression of Gag in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Gag

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

TRE3GV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-empty

Plasmid

#221332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

6xHis-3xFKBP empty scaffold

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRAB14-HA

Plasmid

#214165

Purpose

Transient expression of RAB14

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

RAB14 (Rab14 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BpBBS

Plasmid

#199468

Purpose

Expression of the biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

Insert

BpBBS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-DmeIF4E_31-248_AD

Plasmid

#148517

Purpose

Bacterial Expression of DmeIF4E_31-248

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4E_31-248

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MTOR

Plasmid

#183311

Purpose

All-in-One CRISPRko system with a guide RNA that targets MTOR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MTOR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRAF

Plasmid

#183273

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRAF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRAF

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CYP19A1

Plasmid

#183280

Purpose

All-in-One CRISPRko system with a guide RNA that targets CYP19A1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CYP19A1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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