We narrowed to 2,368 results for: pcas9

1. pTox-EGFPsite1_NGA-PAM (BPK754)

   Plasmid

   #181747

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
   

   Use

   Selection plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173205

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-sgRNA_Ago1_3

   Plasmid

   #73535

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pnCas9-miniSdd3-PBE

   Plasmid

   #204857

   Purpose

   For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd3-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

   Plasmid

   #199253

   Purpose

   Expression construct encoding the high specificity SpCas9-KA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT-9251

   Plasmid

   #124226

   Purpose

   Bacterial SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR/TetA

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-sgRNA_Ago1_4

   Plasmid

   #73536

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CMV-miniSdd7-BE4max-BlastR

    Plasmid

    #204851

    Purpose

    For cytosine base editing using miniSdd7 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE-Plus

    Plasmid

    #214079

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACBE

    Plasmid

    #204607

    Purpose

    Mammalian expression of adenine-to-cytosine base editor ACBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Promoter

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK341

    Plasmid

    #192640

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dSpRY has s…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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