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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Peroxisomes-0

Plasmid

#54501

Purpose

Localization: Peroxisomes, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

EGFP-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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DL146_plenti_TRE_GFP-iPAK4

Plasmid

#187446

Purpose

Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNFkB:eGFP

Plasmid

#44922

Depositor

John Rawls

Article

Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

Insert

EGFP

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-mCherry

Plasmid

#178583

Purpose

Cre-dependent expression of mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

mCherry

Use

AAV

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Mutation

GFP has 20 amino acids deleted from the C terminu…

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLGB30

Plasmid

#126620

Purpose

Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMEH365 Hspa8 HA

Plasmid

#168219

Purpose

Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Hspa8

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMEH380 VSV-G HA

Plasmid

#168222

Purpose

Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

VSV-G

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCLucf

Plasmid

#37328

Depositor

John Schiller

Article

Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

Inserts

Luciferase (luciferase Fly)
GFP

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc13

Plasmid

#198283

Purpose

Produces PVCs lacking tail fibers

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc13

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.MTRIA-OT

Plasmid

#184594

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human synapsin promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFS554

Plasmid

#242854

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW3 promoter

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFS553

Plasmid

#242853

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW2 promoter

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

6xHis-tag

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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