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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

Plasmid

#174620

Purpose

PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

Depositor

Volker Busskamp

Article

Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

Insert

enhanced green fluorescent protein

Use

Piggybac

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-mCardinal-P2A-B19L Flp-in vector

Plasmid

#178519

Purpose

For making TRE-CB mouse line in Tigre locus

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCardinal-P2A-B19L

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p194-RV-CAG-dio-mScarlet-T2A-Brn2

Plasmid

#204741

Purpose

Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Brn2

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183519

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Has Service

AAV8

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

Plasmid

#196889

Purpose

Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

lyn-mScarlet

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mScarlet-MCS-polyA

Plasmid

#174023

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mScarlet

Use

Zebrafish knock-in tagging

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156410

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

mutant KRAS4A-G12V

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

Plasmid

#163988

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1457A

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

Plasmid

#163990

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-hsChRmine-oScarlet-WPRE

Plasmid

#183526

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet

Use

AAV

Mutation

H33R

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-mScarlet- hRec8(297-506)-mNeonGreen

Plasmid

#174717

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Rec8

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Rec8 (REC8 Human)

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

297-506

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

Plasmid

#169737

Purpose

Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72L-T2A-GFP

Plasmid

#163784

Purpose

Expression of R72L CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R72L

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Mutation

mutant KRAS4A-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 SNP (plus strand)

Plasmid

#91851

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0346)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 SNP (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs61839660 C>T

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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