We narrowed to 13,947 results for: cas9

1. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112924

   Purpose

   piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Piggybac transposon vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCas9-sgVRK1 #4-Blast

   Plasmid

   #199647

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ-dpcoCas9-SunTag

   Plasmid

   #158410

   Purpose

   CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-Cas9-2A-GFP

   Plasmid

   #170159

   Purpose

   Express Cas9 and GFP upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   Cas9
   

   Use

   Synthetic Biology; Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas9-D3A

   Plasmid

   #78256

   Purpose

   Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

   Plasmid

   #110302

   Purpose

   Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Expression

   Mammalian

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gephyrin (px330:2-guideCas9)

   Plasmid

   #169432

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-G9a-Hygro

    Plasmid

    #104408

    Purpose

    transient expression of dCas9-G9a fusion protein

    Depositor

    Stephan Beck

    Insert

    G9a (EHMT2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-J6-dCas9-10XGP41-J9

    Plasmid

    #202005

    Purpose

    MoClo level 0 dCas9-10XGP41 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-10XGP41
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GS linkers

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ezh2[SET]-dCas9

    Plasmid

    #100087

    Purpose

    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    Ezh2[SET] (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Ezh2[SET] includes aa 482-746

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-CRY2Clust-BRD4ΔN

    Plasmid

    #200968

    Purpose

    induction of phase-separated condensate mediated by BRD4dN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    BRD4dN
    

    Expression

    Bacterial and Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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