We narrowed to 76,945 results for: rest

1. HyWnt3-ΔRep:Luc

   Plasmid

   #192987

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Expression

   Mammalian

   Mutation

   Repressor element deleted

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-pSP172BSSPE-Swa1::HA-RfA

   Plasmid

   #186404

   Purpose

   Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-pSP172BSSPE-Swa1::RfA-ECFP

   Plasmid

   #186402

   Purpose

   Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-pSP172BSSPE-Swa1::venus-RfA

   Plasmid

   #186398

   Purpose

   Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-pSP172BSSPE-Swa1::RfA-HA

   Plasmid

   #186405

   Purpose

   Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-CfANLN_sgRNA

   Plasmid

   #183880

   Purpose

   pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrichoGate-17

   Plasmid

   #160717

   Purpose

   TrpC Terminator bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
   

   Use

   Synthetic Biology

   Mutation

   Incorporation of BsaI restriction sites

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTurquoise2-RnBarr2

   Plasmid

   #137798

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-myc-Rem2 S69A/S241A/S308A/S334A

   Plasmid

   #51594

   Purpose

   expresses RNAiR myc-tagged Rem2 S-A mutant

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

   Insert

   Rem2 (Rem2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Serines 69, 241, 308, 334 to Alanines; also RNAi …

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-TetOne-Puro-LUC

    Plasmid

    #218630

    Purpose

    This plasmid contains luciferase under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LUC (LOC116160065 )
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

    Plasmid

    #122202

    Purpose

    Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

    Depositor

    Raphael Gaudin

    Article

    Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

    Inserts

    HBc from HBV AYW
    mNeonGreen
    

    Tags

    GGSGGSGGS linker

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. (133) pcDNA3.1-Nup62-Flag-EPEA

    Plasmid

    #160744

    Purpose

    Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    Nup62-Flag-EPEA
    

    Tags

    EPEA and Flag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSGV1-1D3-28Z.1-3 mut

    Plasmid

    #107227

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs mutated

    Depositor

    James Kochenderfer, Steven Rosenberg

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z. 1-3 mut
    

    Use

    Retroviral

    Tags

    None

    Mutation

    None

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSGV-1D3-28Z All ITAMs intact

    Plasmid

    #107226

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs intact

    Depositor

    James Kochenderfer, Steven Rosenberg

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z All ITAMs intact
    

    Use

    Retroviral

    Tags

    None

    Mutation

    None

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEVOL-NBK-1

    Plasmid

    #207639

    Purpose

    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    

    Expression

    Bacterial

    Mutation

    The evolved synthetase NBK-1 contains mutations Y…

    Promoter

    araBAD

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. elavl3:hArr-TEV;he1.1:YFP

    Plasmid

    #218855

    Purpose

    D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-tTRE-scFv-SunTag

    Plasmid

    #193138

    Purpose

    Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    scFv-SunTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Mutation

    G12V

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLAG-Tetherin

    Plasmid

    #41070

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only