162,999 results

1. AbVec2.0-IGHG2

   Plasmid

   #99576

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype

   Depositor

   Hedda Wardemann

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

   Plasmid

   #197028

   Purpose

   AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY2olig-PR

   Plasmid

   #218917

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-PR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2olig-GR

   Plasmid

   #218919

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCA

   Plasmid

   #17363

   Depositor

   Stephen Goff

   Article

   Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

   Insert

   MMLV proviral DNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. σ1-mCherry

   Plasmid

   #186578

   Purpose

   Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP1S1 (AP1S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PresentER-SIINFEKL (mCherry)

   Plasmid

   #102945

   Purpose

   PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   SIINFEKL (OVAL Chicken, Synthetic)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   OVA257-264

   Promoter

   LTR

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pUAP1

    Plasmid

    #63674

    Purpose

    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

    Depositor

    Nicola Patron

    Article

    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

    Insert

    RFP cloning selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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