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Plasmid#63088PurposeAdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2DepositorInsertCCUG 1200x
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Hist4HG-N-18
Plasmid#62757PurposeLocalization: Histones, N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertHist4HG (HIST1H4G Human)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCMV3
Plasmid#85711PurposeExpression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequencesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterMIEPmouse CMV MIEPAvailable sinceApril 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM10-GW-TurboID-V5
Plasmid#196283PurposeGateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic fliesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TC9pAB
Plasmid#71310Purposeused as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsIDepositorInsertCas9
UseIn vitro transcriptionTagsNLSExpressionMutationPromoterT7Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAf
Plasmid#54323PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAr
Plasmid#54324PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV Nup62-V5
Plasmid#175116PurposeLentiviral expression of mouse Nup62-V5DepositorInsertNup62 (Nup62 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-FLP-PGK-Neo
Plasmid#108544PurposeLentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoterDepositorInsertsflp recombinase
Neomycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-Cyto-TurboID
Plasmid#149411PurposeMuscle-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertCyto-TurboID
UseAAVTagsV5ExpressionMutationPromotertMCKAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLenti_P2A_Blast
Plasmid#211365PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-galK
Plasmid#193727PurposeContains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galKDepositorInsertgalK (galK E. coli)
UseTagsExpressionBacterialMutationPromoterJ23113Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Profilin WT
Plasmid#53509DepositorInsertprofilin (chic Fly)
UseTagstdtomatoExpressionInsectMutationPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1/MBP-His
Plasmid#38008DepositorInsertMBP (Maltose binding protein)
UseBaculovirus expressionTags6xHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-cyScarlet
Plasmid#191260PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarletDepositorInsertcyScarlet
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorInserthuMb2Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_HES4
Plasmid#124072PurposeHES4 with normal stop codonDepositorInsertHES4 (HES4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-NatMx
Plasmid#158563PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
UseTags5xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-m5HT6-EGFP
Plasmid#200443PurposeLentiviral expression of mouse m5HT6-EGFPDepositorInsert5HT6-EGFP
UseLentiviralTagsEGFPExpressionMutationPromoterEF1αAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-F979A/N980A
Plasmid#28142DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationF979A and N980APromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pY129 (pcDNA3.1-U6-huMb3Cpf1)
Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Lifeact-7
Plasmid#54671PurposeLocalization: Actin, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsmPA-GFP-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-2xSV40pA-F-td-sfGFP
Plasmid#133572PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTre-tight-SV40-luciferase (496)
Plasmid#65489PurposeInducible expression of coding/noncoding genesDepositorInsertSV40-luciferase
UseLuciferaseTagsExpressionMammalianMutationPromotertight Tre promoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-3xSV40pA-F-td-sfGFP
Plasmid#133573PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-3xSV40pA-L-td-sfGFP
Plasmid#133577PurposeCAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJK645
Plasmid#71700PurposeProduces E. coli thioredoxin (EcTrxA)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-YFP-Eed
Plasmid#82512PurposeLentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.DepositorInsertembryonic ectoderm development
UseLentiviralTagsHaloTagExpressionMammalianMutationinsert contains AA39-441 of EeDPromotercmvAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-td-sfGFP
Plasmid#133576PurposeCAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAG416GAL-3x-uC-ccdB-6stop
Plasmid#203514PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-2x-uA-ccdB-6stop
Plasmid#203517PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK1 -pFlp-18::mito4x-GCaMP6f
Plasmid#194969PurposeFluorescent reporter of mitochondrial matrix Ca2+.DepositorInsert4 repeats of Cox8 presequence plus GCaMP6f
UseTagsExpressionWormMutationPromoterFlp-18Available sinceJune 21, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits