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Showing: 1201 - 1220 of 17715 results

  7. m6A-Tracer-NES

    Plasmid
    #159607
    Purpose
    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    m6A-Tracer-NES
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159607-plasmid-map-sequence-id-311957

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  8. pCD/NL-BH∆1

    Plasmid
    #41791
    Purpose
    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev
    Depositor
    Jakob Reiser
    Article
    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13
    Insert
    HIV-1 Gag/Pol and RRE sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    41791-plasmid-map-sequence-id-171188

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  12. HIV-1 LTR-gfp

    Plasmid
    #115809
    Purpose
    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
    Depositor
    Jeremy Luban
    Article
    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.
    Insert
    gfp and ngfr
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    HIV1 LTR
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    115809-plasmid-map-sequence-id-232377

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  15. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Insert
    RNMT (RNMT Human)
    Use
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112708-plasmid-map-sequence-id-217040

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  16. AA151

    Plasmid
    #215938
    Purpose
    Fragmid fragment: (guide cassette) for dual expression sgRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215938-plasmid-map-sequence-id-431418

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  18. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Use
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  19. pNB2289

    Plasmid
    #113396
    Purpose
    Galactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiae
    Depositor
    Suzanne Sandmeyer
    Insert
    Red fluorescent protein, codon optimized for S. cerevisiae
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113396-plasmid-map-sequence-id-218741

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Showing: 1201 - 1220 of 17715 results