We narrowed to 5,551 results for: SPI

1. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME nls EGFP WPRE bGH pA (JDW 488)

   Plasmid

   #224540

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-EGFP-WPRE-bGH-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pME nls BFP FLAG WPRE bGH pA (JDW 484)

   Plasmid

   #224539

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mTagBFP2-FLAG_WPRE-bGH-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-WPRE-bGH-pA (JDW 1221)

   Plasmid

   #224527

   Purpose

   A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   WPRE-bGH
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E lexOP-c-fos min pro (JDW 1254)

   Plasmid

   #224477

   Purpose

   Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexOp
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p5E-dr_Unc (JDW 1365)

   Plasmid

   #224482

   Purpose

   Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Unc-503 promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

   Plasmid

   #224486

   Purpose

   Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-V5-n2StayGoldC4
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-myr-mTagBFP2-Flag (JDW 1183)

   Plasmid

   #224492

   Purpose

   Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   myr-mTagBFP2-Flag
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Lifeact-mCherry-3xHA (JDW 1321)

   Plasmid

   #224493

   Purpose

   Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mCherry-3xHA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-mCerulean3-TUBB1A (JDW 1367)

    Plasmid

    #224503

    Purpose

    Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean3-TUBB1A
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-TAEL (JDW 1324)

    Plasmid

    #224508

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TAEL
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-Myc-BioID2-MCS1 (JDW 1095)

    Plasmid

    #224510

    Purpose

    Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BioID2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-Myc-BioID2-MCS2 (JDW 1209)

    Plasmid

    #224511

    Purpose

    A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BioID2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME nls EGFP-Cre (JDW 1329)

    Plasmid

    #224515

    Purpose

    A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME iCre-I (JDW 1203)

    Plasmid

    #224520

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    iCre-I
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3E-H2A_mCherry_SV40pA (JDW 967)

    Plasmid

    #224528

    Purpose

    A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

    Plasmid

    #224532

    Purpose

    A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E EFS-rtTA/rtTA3 (JDW 1214)

    Plasmid

    #224533

    Purpose

    A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

    Plasmid

    #224534

    Purpose

    A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge