We narrowed to 24,460 results for: Spr

1. pRha-tns

   Plasmid

   #170630

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTnsABC
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1433

   Plasmid

   #129523

   Purpose

   pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9 (Other)
   

   Use

   Plasmodium, rodent-infectious species

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAra-tns

   Plasmid

   #170632

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTnsABC
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM-HypaCas9-D1135E

   Plasmid

   #154930

   Purpose

   aTc inducible HypaCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   HypaCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N692A/M694A/Q695A/H698A/D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM-Cas9HF1-D1135E

   Plasmid

   #154924

   Purpose

   aTc inducible Cas9HF1 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9HF1-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Mutation

    E993A

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Mutation

    E993A

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG3B-U6SC

    Plasmid

    #134753

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-35SC

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BicC.Ca9.R

    Plasmid

    #168295

    Purpose

    Expresses Cas9 in Drosophila late germline cells

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    BicC.Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPbU6_hdhfr/yfcu_Cas9

    Plasmid

    #216423

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCIPpay-BFP reporter

    Plasmid

    #154096

    Purpose

    Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    P2A-tagBFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCIP-hTRAC

    Plasmid

    #154094

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting human TRAC locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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