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Plasmid#108747PurposeExpresses N-terminus to aa27 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorInserthuman PDS5B (PDS5B Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65455PurposeCan be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinasesDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI
Plasmid#169097PurposeCas9 and Citrine expression in transfected cellsDepositorInsertCas9-2A-Citrine
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)
Plasmid#140000PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFPDepositorInserthuman codon optimized CBE4max xCas9(3.7) with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]
Plasmid#196885PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA
Plasmid#108735PurposeExpresses N-terminus to aa256 of Cre recombinase fused to FRB CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#108770PurposeExpresses aa397 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA
Plasmid#108789PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108790PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050
Plasmid#180644PurposeMammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.DepositorInsertULK1 (ULK1 Human)
UseTagsOne-Strep-Flag (OSF)ExpressionMammalianMutationResidues 833-1050PromoterpCAGAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP
Plasmid#153008PurposeExpresses glucagon peptide on mammalian cell surfaceDepositorInsertGlucagon-ICAM1-pre-mGRASP
UseTags3x HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA
Plasmid#108743PurposeExpresses N-terminus to aa256 of Cre recombinase fused to ABI CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA
Plasmid#108744PurposeExpresses aa257 to C-terminus of Cre recombinase fused to PYL CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA
Plasmid#108771PurposeExpresses N-terminus to aa172 of VCre recombinase fused to GID1 CIDDepositorInsertVCre recombinase fragment N-terminus to aa172
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA
Plasmid#108772PurposeExpresses aa173 to C-terminus of VCre recombinase fused to GAI CIDDepositorInsertVCre recombinase fragment aa173 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin
Plasmid#195101PurposeVector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertCTCF (CTCF Human)
UseTagsAID, HA, and eGFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only