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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Tags

6x His tag

Expression

Bacterial

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version1

Plasmid

#197937

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1delLRR_O

Plasmid

#147117

Purpose

Mammalian Expression of HsNxf-1-delLRR

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-delLRR (NXF1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-INS-sg

Plasmid

#194719

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (INS Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.108

Plasmid

#184977

Purpose

Express -Eco1 LYP1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12

Expression

Yeast

Mutation

LYP1 donor E27stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.106

Plasmid

#184975

Purpose

Express -Eco1 CAN1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12

Expression

Yeast

Mutation

CAN1 donor G444stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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