We narrowed to 2,306 results for: puc19

1. qTAG-C-sTagRFP-Puro

   Plasmid

   #207723

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEF1α-Cre-2A-mTagBFP2

   Plasmid

   #207746

   Purpose

   Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-mTagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDT-sgRNA

   Plasmid

   #138271

   Purpose

   Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   sgBG, sgTS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAureus-TnCAM

   Plasmid

   #199807

   Purpose

   vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

   Depositor

   Steve Salipante

   Article

   Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

   Insert

   transposon cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-KO-Blast

   Plasmid

   #227263

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-mScarlet3

   Plasmid

   #219640

   Purpose

   Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIN-Ri

   Plasmid

   #197784

   Purpose

   Level 2 destination vector: pMIN-Ri backbone

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIVT-Flp-2A-BleoR

    Plasmid

    #232752

    Purpose

    IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Inserts

    FlpO
    BleoR
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-C-mScarlet-Blast

    Plasmid

    #207711

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid

    #101785

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Insert

    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. qTAG-N-Blast-miniTurbo

    Plasmid

    #207681

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAB1620 hU6-BpiI-Cas13bt3-DR

    Plasmid

    #176315

    Purpose

    hU6-BpiI-Cas13bt3-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt3 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIVT-iCre

    Plasmid

    #232773

    Purpose

    IVT template for making modRNA encoding iCre

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    iCre
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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