We narrowed to 7,456 results for: Lif

1. pHD-FlpTag-DsRed-HDR-ph0

   Plasmid

   #231900

   Purpose

   dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

   Depositor

   Alexander Borst

   Article

   Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

   Insert

   Frt-Frt14-SD-GFP-SA-Frt-Frt14
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEW107

   Plasmid

   #232796

   Purpose

   COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

   Insert

   COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
   

   Use

   Tags

   His6-3xFLAG, Twinstrep

   Expression

   Insect

   Mutation

   ySet1(762-1080)

   Promoter

   polyhedrin promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-1_Rluc-Y39x-Fluc

   Plasmid

   #233615

   Purpose

   Expression of Rluc-Fluc reporter gene containing 39xY

   Depositor

   Shintaro Iwasaki

   Article

   Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

   Insert

   Rluc-Y39x-Fluc
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-GEM

   Plasmid

   #187648

   Purpose

   Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Insert

   PfV
   

   Use

   AAV

   Tags

   HaloTag and SNAP-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP-GFP-Evectin-2

   Plasmid

   #221754

   Purpose

   Stable expression of Evectin-2 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PLEKHB2 (PLEKHB2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem PLEKHB2(PH domain) (amino acids 1-110)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIP-GFP-PLCd1(PHD)

   Plasmid

   #221753

   Purpose

   Stable expression of GFP-PLCd1(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PLCd1 (PLCD1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–175

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIP-GFP-2xFYVE

   Plasmid

   #221750

   Purpose

   Stable expression of GFP-2xFYVE in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   tandem HRS FYVE domain
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BoxB-MS2 adRNA (RAB7A-GAC site)

   Plasmid

   #170149

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(GAC)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MS2-adRNA (RAB7A-GAC site)

   Plasmid

   #170140

   Purpose

   AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-RAB7A(GAC)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-HsProm1s1(W795R)-StayGold

    Plasmid

    #210822

    Purpose

    Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Changed Trp-795 to Arg

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-HsProm1s1-TwinStrep-His

    Plasmid

    #210816

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-HsProm1s1-StayGold

    Plasmid

    #210821

    Purpose

    Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TOPO-nmba

    Plasmid

    #184821

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nmba

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nmba (nmba Zebrafish)
    

    Use

    Template for synthesis of nmba probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Halo-MDC1 PST deletion

    Plasmid

    #207109

    Purpose

    pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag-MDC1 PST deletion
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Halo-MDC1 BRCT domain deletion

    Plasmid

    #207110

    Purpose

    pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag-MDC1 BRCT domain deletion
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL1313

    Plasmid

    #217955

    Purpose

    Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

    Plasmid

    #179717

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3H-α-synuclein

    Plasmid

    #205714

    Purpose

    Mammalian expression of 3H-alpha-synuclein

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. α-synuclein-3H

    Plasmid

    #205715

    Purpose

    Mammalian expression of alpha-synuclein-3H

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-αS-2

    Plasmid

    #205706

    Purpose

    Mammalian expression of alpha-synuclein tandem dimer

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein tandem dimer (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQE-80L 2xHis-Rab12 Q101L

    Plasmid

    #207347

    Purpose

    Bacterial expression of Rab12

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    Rab12 (RAB12 Human)
    

    Use

    Tags

    2xHis

    Expression

    Bacterial

    Mutation

    Q101L

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-Best1-N.Txnip

    Plasmid

    #206348

    Purpose

    AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N terminal half (1-228aa)

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-Best1-sC.Txnip

    Plasmid

    #206351

    Purpose

    AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Short C terminal half (255-397aa)

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-Best1-nt.Txnip(C247S)(1-320aa)

    Plasmid

    #206352

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    (1-320aa), C247S

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-RO1.7-dnHIF

    Plasmid

    #206343

    Purpose

    AAV plasmid expressing dominant negative HIF1A in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

    Insert

    dominant negative hif1a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    dominant negative HIF1A

    Promoter

    1.7kb human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-RedO-nt.Txnip(C247S)(1-301aa)

    Plasmid

    #206354

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    (1-301aa), C247S

    Promoter

    human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

    Plasmid

    #190876

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode210
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-EGFP barcode-9-SV40 polyA

    Plasmid

    #190873

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge