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pCIneo-lambdaN-HA-HsAGO3_C

Plasmid

#146084

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD113

Plasmid

#121912

Purpose

Charge-Introduced NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

S110K, I111K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA056

Plasmid

#128516

Purpose

Expresses Xylanase B+GUS

Depositor

Marcos Alcocer

Article

Alcocer et al Appl Microbiol Biotechnol. 2003 Feb;60(6

Insert

Xylanase B + uidA

Expression

Bacterial

Promoter

Histone 4B

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA028

Plasmid

#128513

Purpose

Expresses Xylanase C+GUS

Depositor

Marcos Alcocer

Article

Alcocer et al Appl Microbiol Biotechnol. 2003 Feb;60(6

Insert

Xylanase C + uidA

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA034

Plasmid

#128514

Purpose

Expresses Xylanase A+GUS

Depositor

Marcos Alcocer

Article

Alcocer et al Appl Microbiol Biotechnol. 2003 Feb;60(6

Insert

Xylanase A +uidA

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE415: pTOP_ACA1_Vc_HOG1 terminator

Plasmid

#122434

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE416: pTOP_CAC1_Vn_HOG1 terminator

Plasmid

#122435

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.33

Plasmid

#62880

Purpose

Bacterial expression of GST-MEG-3

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GST-MEG-3

Tags

GST

Expression

Bacterial

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.34

Plasmid

#62881

Purpose

Bacterial expression of GST-MEG-4

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GST-MEG-4

Tags

GST

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.31

Plasmid

#62879

Purpose

Bacterial expression of GST-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GST-MEG-1

Tags

GST

Expression

Bacterial

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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LK444K18

Plasmid

#44620

Depositor

Robert Oshima

Article

Kulesh et al Mol Cell Biol. 1989 Apr;9(4):1553-65.

Insert

K18 (KRT18 Human)

Expression

Mammalian

Promoter

human Beta-Actin

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
peceK8

Plasmid

#44621

Depositor

Robert Oshima

Article

Kulesh et al Mol Cell Biol. 1989 Apr;9(4):1553-65.

Insert

K8 (KRT8 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pBS TRPS1

Plasmid

#19197

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

TRPS1 (TRPS1 Chicken)

Expression

Bacterial

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
pLIB-RSP1

Plasmid

#176690

Purpose

Expression of Chlamydomonas reinhardtii RSP1 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP1

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP2

Plasmid

#176691

Purpose

Expression of Chlamydomonas reinhardtii RSP2 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP2

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP9

Plasmid

#176697

Purpose

Expression of Chlamydomonas reinhardtii RSP9 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP9

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP10

Plasmid

#176698

Purpose

Expression of Chlamydomonas reinhardtii RSP10recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP10

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP11

Plasmid

#176699

Purpose

Expression of Chlamydomonas reinhardtii RSP11 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP11

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP12

Plasmid

#176700

Purpose

Expression of Chlamydomonas reinhardtii RSP12 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP12

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP16

Plasmid

#176701

Purpose

Expression of Chlamydomonas reinhardtii RSP16 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP16

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP23

Plasmid

#176702

Purpose

Expression of Chlamydomonas reinhardtii RSP23 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP23

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge