We narrowed to 5,620 results for: pcas

1. pCAGGS-MTS(S17A)-EGFP-V5

   Plasmid

   #200928

   Purpose

   expresses MTS(S17A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S17A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS-MTS(S23A)-EGFP-V5

   Plasmid

   #200929

   Purpose

   expresses MTS(S23A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   MTS amino acid 23 mutated to Alanine

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-MTS(S27A)-EGFP-V5

   Plasmid

   #200930

   Purpose

   expresses MTS(S27A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S27A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-MTS(S31A)-EGFP-V5

   Plasmid

   #200931

   Purpose

   expresses MTS(S31A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S31A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-MTS(S34A)-EGFP-V5

   Plasmid

   #200932

   Purpose

   expresses MTS(S34A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S34A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS--MTS(S43A)-EGFP-V5

   Plasmid

   #200933

   Purpose

   expresses MTS(S43A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S43A in MTS

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-MTS(S17A)-TFAM-V5

   Plasmid

   #203668

   Purpose

   expresses MTS(S17A)-TFAM-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S17A

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-MTS(S43A)-TFAM-V5

   Plasmid

   #203669

   Purpose

   expresses MTS(S43A)-TFAM-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S43A

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-FLAG-BubR1-D911A

   Plasmid

   #200815

   Purpose

   expresses BubR1-D911A in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Aspartic acid 911 to Alanine

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-MTS(S6A)-EGFP-V5

    Plasmid

    #200817

    Purpose

    expresses MTS(S6A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    Changed Serine 6 to Alanine in MTS

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-MTS(S12A)-EGFP-V5

    Plasmid

    #200818

    Purpose

    expresses MTS(S12A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S12A in MTS

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG_mCherry-ATG13 HORMA (1-191aa)

    Plasmid

    #223759

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    ATG13 HORMA (ATG13 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-191 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG/VPS15(R36D)-TSF

    Plasmid

    #232913

    Purpose

    Mammalian Expression

    Depositor

    James Hurley

    Insert

    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R36D

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG/VPS34(W885D/Y884D)

    Plasmid

    #232918

    Purpose

    Mammalian Expression

    Depositor

    James Hurley

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W885D/Y884D

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-GluA2-Fab-HC-Halo

    Plasmid

    #230061

    Purpose

    Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab heavy chain fragment
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG CAPN7 V18D-OSF

    Plasmid

    #193042

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG CAPN7 L61D-OSF

    Plasmid

    #193043

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    L61D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG CAPN7 F98D-OSF

    Plasmid

    #193044

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    F98D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCA528 CHMP1B 143-199

    Plasmid

    #108255

    Purpose

    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1B residues 143-199 (CHMP1B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCA528 CHMP4A 153-222

    Plasmid

    #108256

    Purpose

    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4A residues 153-222 (CHMP4A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCA528 CHMP4C 156-233

    Plasmid

    #108257

    Purpose

    Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C residues 156-233 (CHMP4C Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCA528 CHMP5 159-222

    Plasmid

    #108259

    Purpose

    Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP5 residues 148-219 (CHMP5 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCA528 CHMP2B 141-213

    Plasmid

    #104595

    Purpose

    Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2B residues 141-213 (CHMP2B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA528 CHMP4B 156-224

    Plasmid

    #104596

    Purpose

    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B residues 156-224 (CHMP4B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge