We narrowed to 9,179 results for: sacs
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Dom34(1-138)
Plasmid#19101DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K82A
Plasmid#19112DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK82APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET50del_SynPspA
Plasmid#223818PurposeBacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage siteDepositorInsertSynPspA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_C2
Plasmid#228158PurposePlasmid contains the secretion signal peptide sequence of the yeast (S. mikatae) MEL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_Q11129_MEL_SACMI:0-18
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_A2
Plasmid#228146PurposePlasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_Q876J2_SPR1_SACU7:0-20
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSET_SynPspA
Plasmid#223812PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage siteDepositorInsertSynPspA
UseTags10xHis and NusAExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET50delCT_Vipp1_dL10Ala
Plasmid#223817PurposeBacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage siteDepositorInsertVipp1
UseTags6xHisExpressionBacterialMutationreplacement of 10 aa in Hinge 2 with AlaPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTPI1-Dsred-URA3
Plasmid#207017PurposeExpresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).DepositorInsertDsRed-Express 2
UseTagsExpressionYeastMutationPromoterpTPI1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-TtrpC
Plasmid#213467PurposeVector containing the TrpC terminator for Golden Gate cloning in the Fg vectorDepositorInsertTerminator of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PgpdA
Plasmid#213466PurposeVector containing the gpdA promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of the glyceraldehyde-3-phosphate dehydrogenase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PtrpC
Plasmid#213465PurposeVector containing the TrpC promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-HFL1-8flag-LEU
Plasmid#207035PurposeExpression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertHFL1
UseTags8xFLAGExpressionYeastMutationPromoterpHFL1Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hygro-Bem1-mCherry-AID
Plasmid#173925PurposePlasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AIDDepositorInsertsPartial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence
UseTagsmCherry- AIDExpressionMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D94 (pAF10C6)
Plasmid#185848PurposeExpressing artificial trans-repressor in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>CYC8>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScGm-rpoB
Plasmid#166979PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistanceDepositorInsertgentamycin-3-acetyltransferase (aac(3)-Ia )
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dom34(1-377)
Plasmid#19100DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E23K
Plasmid#19105DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE23KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E149K
Plasmid#19107DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE149KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 F218A
Plasmid#19108DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationF218APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E261K
Plasmid#19109DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE261KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 Y300A
Plasmid#19110DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationY300APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K45A
Plasmid#19111DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK45APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 41kDa
Plasmid#201342PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
